Simple method for demonstration of differential colony morphology of plasmid-associated virulent clones of Yersinia enterocolitica.
نویسندگان
چکیده
Virulent (plasmid-associated) strains of Yersinia enterocolitica grown on RPMI 1640 agar (RPMI-1640 medium [Flow Laboratories] with 40 mM HEPES and 1.5% [wt/vol] purified agar [Difco]) dissociated into small and large colonies. The autoagglutination test (a marker of virulence) is regularly positive with small colonies and negative with large colonies. Avirulent Y. enterocolitica strains gave only large colonies on RPMI 1640 agar.
منابع مشابه
Simple assay of calcium dependency for virulent plasmid-bearing clones of Yersinia enterocolitica.
A simplified procedure to detect the calcium dependency of virulent plasmid-bearing strains of Yersinia enterocolitica was developed. A low-calcium, agarose-based medium of brain heat infusion with added magnesium effectively differentiated plasmid-bearing and plasmidless isolates. Further, the expression of calcium dependency in plasmid-bearing strains of Y. enterocolitica as measured by the a...
متن کاملVirulence Plasmid (pYV)-Associated Expression of Phenotypic Virulent Determinants in Pathogenic Yersinia Species: A Convenient Method for Monitoring the Presence of pYV under Culture Conditions and Its Application for Isolation/Detection of Yersinia pestis in Food
In Yersinia pestis, Y. pseudotuberculosis, and Y. enterocolitica, phenotypic expression of virulence plasmid (pYV: 70-kb)-associated genetic determinants may include low-calcium response (Lcr, pinpoint colony, size = 0.36 mm), colony morphology (size = 1.13 mm), crystal violet (CV) binding (dark-violet colony), Congo Red (CR) uptake (red pinpoint colony, size = 0.36 mm), autoagglutination (AA =...
متن کاملDirect detection and isolation of plasmid-bearing virulent serotypes of Yersinia enterocolitica from various foods.
A procedure was developed for direct detection, isolation, and maintenance of plasmid-bearing virulent serotypes of Yersinia enterocolitica from different food sources. Plasmid-bearing virulent strains of Y. enterocolitica representing five serotypes were simultaneously detected and isolated from enriched swab samples of artificially contaminated pork chops, ground pork, cheese, and zucchini, u...
متن کاملارتباط حضـور پلاسمیــــد بیماریزا با تستهای سرولوژیکی در ایزوله های Yersinia enterocolitica جدا شده از انسان
Pathogenicity of nine human Yersinia enterocolitica isolates of Iran were tested by serological tests and molecular approaches. Of these nine isolates two harbored a plasmid (1478 & 1479). These two isolates showed differ...
متن کاملEvaluation of DNA colony hybridization and other techniques for detection of virulence in Yersinia species.
The virulence of yersiniae varies according to (i) species and biotype and (ii) possession of a 67- to 72-kilobase virulence plasmid. Y. pestis, Y. pseudotuberculosis, and biotypes 1B, 2, 3, 4, and 5 of Y. enterocolitica are inherently virulent but express full virulence only when in possession of a virulence plasmid. Other Yersinia species and biotypes 1A and 3B of Y. enterocolitica are seldom...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 17 3 شماره
صفحات -
تاریخ انتشار 1983